RNA
extraction——RNA quality inspection——Isolate mRNA using oligo dT
magnetic beads——Fragment mRNA sequences——Reverse transcription into
cDNA——End repair and add adapter——Fragment sorting and PCR
amplification——Sequencing—— Data quality control——Bioinformatics
analysis
RNA extraction——RNA quality inspection——Isolate mRNA using oligo dT magnetic beads——Fragment mRNA sequences——Reverse transcription into cDNA——End repair and add adapter——Fragment sorting and PCR amplification——Sequencing—— Data quality control——Bioinformatics analysis
