Resequencing is used to obtain the variation information by the means of high-throughput sequencing. Fungi whole genome resequencing refers to aligning the sequencing reads to the reference genome (sequence similarity between the tested stain genome and the reference genome ≥ 98%) to retrieve the SNP, InDel, CNV and SV.
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DNA amount* mentioned above is just for once experiment. In fact, three times DNA is recommended in library construction.