SSR markers Development
Microsatellite is also called Short Tandem Repeats（STRs）or simple sequence repeats (SSR). It is widely distributed in all eukaryote genomes. On average, there is one microsatellite site per 6 Kb (Beckmann & Weber, 1992). With the increase of the base length of each repeat unit, the total number of microsatellite sites decreased. It has the characteristics of rapid mutation rate and high polymorphism.
Genomic library from the whole genomic DNA of multiple mixed samples is constructed. The enrichment of core regions and conservative sequence regions at both ends of SSR is achieved by using probes, then the second generation sequencing platform and bioinformatic analysis are applied to acquire of polymorphic SSR sequence and design primers of SSRs.
Applications:
Genetic map construction, Germplasm identification, Genetic diversity analysis, Gene location, Quantitative trait loci (QTL) analysis
