Full Length Transcriptome Sequencing
Full
length transcriptome sequencing (Iso-Seq) based on PacBio sequencing
platform can directly obtain the full-length sequence of mRNA and can
more accurately interpret the structural information of mRNA. Full
length transcriptome sequencing technology can overcome the problem of
short assembly sequence and incomplete information of transcripts for
non-reference genomic species. It can obtain full-length mRNA sequence,
analyze homologous isomer, single base mutation detection, alternative
splicing analysis, homologous gene recognition and allele recognition.
Project Advice
Full length
transcriptome sequencing is suitable for a single sample or multiple
samples. For species with reference genome, Iso-Seq can achieve the
alternative splicing and other gene structure analysis accurately. For
species without reference genome, Iso-Seq can also help the assembly of
RNA-seq.
