国产青榴社区91精品,久久成人精品免费播放,久久精品人人做人人试看

Home>(Research) Service>Transcriptome & Epigenome Sequencing>Transcriptome>Eukaryotic mRNA Sequencing with Reference Genome

Eukaryotic mRNA Sequencing with Reference Genome

FAQ

  •  A brief introduction of the construction of eukaryotic transcriptome library


    1) mRNA was separated by magnetic beads for polyA structure of mRNA

    2) mRNA fragmentation

    3) The base T is replaced by U during the second strand of cDNA synthetization to achieve the purpose of strand specific library.

    4) 5 'end repair, 3' end add “A”

    5) Add adapters

    6) Collect fragments with specific length, generally 300bp

    7) PCR

    8) Sequencing


  • How to select appropriate genes for qRT-PCR verification?


     Firstly, according to the enrichment results of GO/KEGG, the genes concerned shall be selected. Then, according to the results of differential expression analysis, the genes with higher fold change value, smaller P value and higher FPKM value shall be verified by qRT-PCR.


主站蜘蛛池模板: 休宁县| 张北县| 汪清县| 巴东县| 和硕县| 上杭县| 古浪县| 疏勒县| 广昌县| 陈巴尔虎旗| 吴旗县| 彝良县| 崇左市| 日照市| 阿拉善右旗| 龙井市| 当涂县| 慈利县| 宁安市| 墨脱县| 渝中区| 静海县| 郸城县| 高雄市| 宁国市| 九江市| 永吉县| 龙陵县| 永济市| 贡嘎县| 奉化市| 三江| 长顺县| 青岛市| 康保县| 翁牛特旗| 神池县| 阳东县| 扶风县| 西峡县| 武夷山市|