RNA
extraction——RNA quality inspection——Remove rRNA sequence——Fragment RNA
sequences——Reverse transcription into cDNA——End repair and add
adapter——Fragment sorting and PCR amplification——Sequencing—— Data
quality control——Bioinformatics analysis
RNA extraction——RNA quality inspection——Remove rRNA sequence——Fragment RNA sequences——Reverse transcription into cDNA——End repair and add adapter——Fragment sorting and PCR amplification——Sequencing—— Data quality control——Bioinformatics analysis