RNA
extraction——RNA quality inspection——Isolate mRNA using oligo dT
magnetic beads——Fragment RNA sequences——Reverse transcription into
cDNA——UMI labled——Purification of ligation products——Fragment sorting
and PCR amplification——Sequencing—— Data quality control——Bioinformatics
analysis
RNA extraction——RNA quality inspection——Isolate mRNA using oligo dT magnetic beads——Fragment RNA sequences——Reverse transcription into cDNA——UMI labled——Purification of ligation products——Fragment sorting and PCR amplification——Sequencing—— Data quality control——Bioinformatics analysis
