Illumina MiSeq
NextSeq??2000
Illumina NovaSeq 6000
PacBio Sequel II
PacBio Sequel
Species requirements: Animals and plants with reference genome
Sample amount requirements: Total RNA amount ≥ 500μg, concentration ≥ 1μg/μl; tissues amount ≥25g; cell samples≥1 × 109 ; immunoprecipitated samples ≥ 200ng