国产青榴社区91精品,久久成人精品免费播放,久久精品人人做人人试看

Home>(Research) Service>Transcriptome & Epigenome Sequencing>Transcriptome>Eukaryotic mRNA Sequencing with Reference Genome

Eukaryotic mRNA Sequencing with Reference Genome

FAQ

  •  A brief introduction of the construction of eukaryotic transcriptome library


    1) mRNA was separated by magnetic beads for polyA structure of mRNA

    2) mRNA fragmentation

    3) The base T is replaced by U during the second strand of cDNA synthetization to achieve the purpose of strand specific library.

    4) 5 'end repair, 3' end add “A”

    5) Add adapters

    6) Collect fragments with specific length, generally 300bp

    7) PCR

    8) Sequencing


  • How to select appropriate genes for qRT-PCR verification?


     Firstly, according to the enrichment results of GO/KEGG, the genes concerned shall be selected. Then, according to the results of differential expression analysis, the genes with higher fold change value, smaller P value and higher FPKM value shall be verified by qRT-PCR.





主站蜘蛛池模板：
温泉县|
彰化县|
丹东市|
敦化市|
静宁县|
辽宁省|
锦州市|
保靖县|
丹巴县|
丹棱县|
垦利县|
长治县|
屏南县|
太谷县|
乡城县|
若羌县|
汾西县|
额济纳旗|
黄平县|
屯昌县|
曲靖市|
松江区|
固安县|
集贤县|
增城市|
北宁市|
林州市|
新巴尔虎右旗|
赤壁市|
济源市|
棋牌|
买车|
微博|
太原市|
阿克|
冀州市|
长沙市|
新源县|
玉溪市|
建阳市|
民乐县|