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RNA Methylation

FAQ

  • What are the common modification sites of m6A methylation?


    m6A tends to be distributed in protein coding sequence (CDS), 3’ untranslated region (3’ UTR), termination codon, splicing site and long exon region of mRNA, and its modification region is highly conserved in human and mouse; m6A is also enriched in microRNA target sites and its adjacent regions.

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  • What are the technical advantages of mRNA methylation sequencing?

    High flexibility: it can directly sequence the transcriptome hypermethylation fragments of any species without reference.

    High precision: it can accurately locate the binding site in the range of 100-200 bases.

    Wide detection range: it covers the methylation region of the whole transcriptome.

    Digital signal: direct quantification and sequencing of methylation fragments, no cross reaction and background noise problems caused by fluorescence analog signals of traditional chip hybridization.





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