国产青榴社区91精品,久久成人精品免费播放,久久精品人人做人人试看

Home> Market activity > Achievements > article shows > article details

Modulation of activated astrocytes in the hypothalamus paraventricular nucleus to prevent ventricular arrhythmia complicating acute myocardial infarction

2020-07-08

Abstract

Background

Sympathetic overactivation after acute myocardial infarction (AMI) contributes to ventricular arrhythmia (VA). Paraventricular nucleus (PVN) of the hypothalamus may play an important role on this context, however, the mechanisms remain unknown. In this study, we investigated whether inhibition of activated astrocytes in the PVN could reduce VA in rats with AMI.

Methods

The anesthetized rats were randomly divided into four groups of sham-operated, AMI, AMI + vehicle and AMI + fluorocitrate (FCA). Electrocardiogram was continuously recorded. RNA sequencing, sympathetic nerve activity (heart rate variability and norepinephrine levels) and ventricular electrical instability (ventricular effective refractory period and ventricular fibrillation inducibility) were measured. Furthermore, brain tissues were extracted to detect expression of inflammatory cytokines (IL-6, and TNF-α), astrocyte and neuro activation.

Results

RNA sequencing analysis showed that functions of differentially expressed genes in the PVN of AMI rats were significantly enriched in immune system- and neuroactive-related pathways, along with enhance expression of cytokines and Glial fibrillary acidic protein (GFAP). We further characterized that astrocytes were activated in PVN and intervention of activation astrocytes by FCA significantly inhibited sympathetic nerve activity and decreased the incidence of VA and ventricular electrical instability in rats with AMI. Moreover, FCA significantly attenuated neurons activation and downregulated expression of inflammatory cytokines in the PVN.

Conclusions

Inhibition of activated astrocytes in the PVN could reduce VA occurrence and improve ventricular electrical instability in AMI rats by central neuro-immune pathway. These findings suggest that astrocytes are a potential target for prevention and treatment of VA complicating AMI.


Text Link:

https://www.sciencedirect.com/science/article/abs/pii/S0167527319358292


主站蜘蛛池模板: 洪洞县| 精河县| 蕲春县| 周宁县| 上杭县| 邵武市| 厦门市| 安阳市| 新平| 江安县| 登封市| 新野县| 永平县| 正定县| 古丈县| 峡江县| 色达县| 巴彦淖尔市| 寿阳县| 湾仔区| 定南县| 敦煌市| 台湾省| 南丰县| 蛟河市| 房山区| 东辽县| 翁牛特旗| 安龙县| 多伦县| 工布江达县| 余江县| 龙海市| 东阿县| 波密县| 保康县| 镇坪县| 多伦县| 通州市| 惠水县| 炉霍县|