国产青榴社区91精品,久久成人精品免费播放,久久精品人人做人人试看

Home> Market activity > Achievements > article shows > article details

Modulation of activated astrocytes in the hypothalamus paraventricular nucleus to prevent ventricular arrhythmia complicating acute myocardial infarction

2020-07-08

Abstract

Background

Sympathetic overactivation after acute myocardial infarction (AMI) contributes to ventricular arrhythmia (VA). Paraventricular nucleus (PVN) of the hypothalamus may play an important role on this context, however, the mechanisms remain unknown. In this study, we investigated whether inhibition of activated astrocytes in the PVN could reduce VA in rats with AMI.

Methods

The anesthetized rats were randomly divided into four groups of sham-operated, AMI, AMI + vehicle and AMI + fluorocitrate (FCA). Electrocardiogram was continuously recorded. RNA sequencing, sympathetic nerve activity (heart rate variability and norepinephrine levels) and ventricular electrical instability (ventricular effective refractory period and ventricular fibrillation inducibility) were measured. Furthermore, brain tissues were extracted to detect expression of inflammatory cytokines (IL-6, and TNF-α), astrocyte and neuro activation.

Results

RNA sequencing analysis showed that functions of differentially expressed genes in the PVN of AMI rats were significantly enriched in immune system- and neuroactive-related pathways, along with enhance expression of cytokines and Glial fibrillary acidic protein (GFAP). We further characterized that astrocytes were activated in PVN and intervention of activation astrocytes by FCA significantly inhibited sympathetic nerve activity and decreased the incidence of VA and ventricular electrical instability in rats with AMI. Moreover, FCA significantly attenuated neurons activation and downregulated expression of inflammatory cytokines in the PVN.

Conclusions

Inhibition of activated astrocytes in the PVN could reduce VA occurrence and improve ventricular electrical instability in AMI rats by central neuro-immune pathway. These findings suggest that astrocytes are a potential target for prevention and treatment of VA complicating AMI.


Text Link:

https://www.sciencedirect.com/science/article/abs/pii/S0167527319358292


主站蜘蛛池模板: 札达县| 博白县| 绿春县| 佛坪县| 随州市| 科技| 海南省| 界首市| 朔州市| 紫阳县| 礼泉县| 谷城县| 吴旗县| 遵化市| 宁乡县| 师宗县| 宜川县| 洱源县| 临猗县| 神农架林区| 嵊泗县| 格尔木市| 剑川县| 曲水县| 贺州市| 江北区| 隆昌县| 高密市| 宁陵县| 方城县| 潜山县| 五寨县| 西昌市| 赣榆县| 涟源市| 拜城县| 遂昌县| 皋兰县| 宜兰市| 会理县| 汉川市|