国产青榴社区91精品,久久成人精品免费播放,久久精品人人做人人试看

Home> Market activity > Achievements > article shows > article details

Transcriptomics Sequencing Provides Insights into Understanding the Mechanism of Grass Carp Reovirus Infection

2018-04-26

Abstract: 

Grass carp is an important aquaculture fish species in China that is affected by severe diseases, especially haemorrhagic disease caused by grass carp reovirus (GCRV). However, the mechanisms of GCRV invasion and infection remain to be elucidated. In the present study, Ctenopharyngodon idellus kidney (CIK) cells were infected with GCRV, harvested at 0, 8, 24, and 72 h post infection, respectively, and then subjected to transcriptomics sequencing. Each sample yielded more than 6 Gb of clean data and 40 million clean reads. To better understand GCRV infection, the process was divided into three phases: the early (0–8 h post infection), middle (8–24 h post infection), and late (24–72 h) stages of infection. A total of 76 (35 up-regulated, 41 down-regulated), 553 (463 up-regulated, 90 down-regulated), and 284 (150 up-regulated, 134 down-regulated) differently expressed genes (DEGs) were identified during the early, middle, and late stages of infection, respectively. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that DEGs were mainly involved in carbohydrate biosynthesis, transport, and endocytosis in the early stage, phagocytosis and lysosome pathways were mainly enriched in the middle stage, and programmed cell death, apoptosis, and inflammation were largely associated with the late stage. These results suggest GCRV infection is a gradual process involving adsorption on the cell surface, followed by endocytosis into cells, transport by lysosomes, and eventually resulted in cell necrosis and/or apoptosis. Our findings provide insight into the mechanisms of grass carp reovirus infection. 

Keywords: 

grass carp; grass carp reovirus; transcriptomics sequencing; phagosome

主站蜘蛛池模板: 白玉县| 凉山| 湘西| 伊通| 方山县| 离岛区| 安达市| 那曲县| 清徐县| 阿城市| 交城县| 京山县| 永宁县| 岫岩| 武陟县| 临城县| 安平县| 郑州市| 临夏县| 南江县| 灵寿县| 阳西县| 开阳县| 常宁市| 临武县| 广州市| 子长县| 名山县| 鹤峰县| 红原县| 周口市| 五莲县| 泰顺县| 新津县| 都兰县| 奉新县| 乡宁县| 宜昌市| 泸西县| 咸丰县| 陆良县|