国产青榴社区91精品,久久成人精品免费播放,久久精品人人做人人试看

Home> Market activity > Achievements > article shows > article details

Tropane alkaloids biosynthesis involves an unusual type Ⅲ polyketide synthase and non-enzymatic condensation

2019-10-17

Abstract

The skeleton of tropane alkaloids is derived from ornithine-derived N-methylpyrrolinium and two malonyl-CoA units. The enzymatic mechanism that connects N-methylpyrrolinium and malonyl-CoA units remains unknown. Here, we report the characterization of three pyrrolidine ketide synthases (PYKS), AaPYKS, DsPYKS, and AbPYKS, from three different hyoscyamine- and scopolamine-producing plants. By examining the crystal structure and biochemical activity of AaPYKS, we show that the reaction mechanism involves PYKS-mediated malonyl-CoA condensation to generate a 3-oxo-glutaric acid intermediate that can undergo non-enzymatic Mannich-like condensation with N-methylpyrrolinium to yield the racemic 4-(1-methyl-2-pyrrolidinyl)-3-oxobutanoic acid. This study therefore provides a long sought-after biosynthetic mechanism to explain condensation between N-methylpyrrolinium and acetate units and, more importantly, identifies an unusual plant type III polyketide synthase that can only catalyze one round of malonyl-CoA condensation.


Text link:

https://www.nature.com/articles/s41467-019-11987-z

主站蜘蛛池模板: 阿合奇县| 吉水县| 巴林右旗| 斗六市| 连平县| 丁青县| 乳山市| 重庆市| 漾濞| 铁岭市| 莎车县| 大安市| 彩票| 霍林郭勒市| 孝昌县| 阳谷县| 保靖县| 平南县| 都昌县| 高雄县| 唐山市| 罗城| 青州市| 随州市| 临江市| 绥阳县| 滦南县| 正宁县| 丰宁| 繁昌县| 咸宁市| 贵南县| 布拖县| 犍为县| 河间市| 曲麻莱县| 湛江市| 天等县| 延庆县| 海丰县| 依兰县|