国产青榴社区91精品,久久成人精品免费播放,久久精品人人做人人试看

Home> Market activity > Achievements > article shows > article details

Effects of different fertilizers on methane emissions and methanogenic community structures in paddy rhizosphere soil

2018-07-26



Abstract


Paddy soil accounts for 10% of global atmospheric methane (CH4) emissions. Many types of fertilizers may enhance CH4 emissions, especially organic fertilizer. The aimof this studywas to explore the effects of different fertilizers on CH4 and methanogen patterns in paddy soil. This experiment involved four treatments: chemical fertilizer (CT), organic fertilizer (OT), mixed with chemical and organic fertilizer (MT), and no fertilizer (ctrl). The three fertilization treatments were applied with total nitrogen at the same rate of 300 kg N ha?1. Paddy CH4, soil physicochemical variables and methanogen communities were quantitatively analyzed. Rhizosphere soil mcrA and pmoA gene copy numbers were determined by qPCR. Methanogenic 16S rRNA genes were identified by MiSeq sequencing. The results indicated CH4 emissions were significantly higher in OT (145.31 kg ha?1) thanMT (84.62 kg ha?1), CT (77.88 kg ha?1) or ctrl (32.19 kg ha?1). Soil organic acidswere also increased by organic fertilization. CH4 effluxes were significantly and negatively related to mcrA and pmoA gene copy numbers, and positively related to mcrA/pmoA. Above all, hydrogenotrophic Methanocella and acetoclastic Methanosaeta

were the predominant methanogenic communities; these communities were strictly associated with soil potassium, oxalate, acetate, and succinate. Application of organic fertilizer promoted the dominant acetoclastic methanogens, but suppressed the dominant hydrogenotrophic methanogens. The transformation in methanogenic community structure and enhanced availability of C substrates may explain the increased CH4 production in OT compared to other treatments. Compared to OT, MT may partially mitigate CH4 emissions while guaranteeing a high rice yield. On this basis, we recommend the local fertilization pattern should change from 300 N kg ha?1 of organic manure to the same level of mixed fertilization. Moreover, we suggest multiple combinations of mixed fertilization merit more investigation in the future.


Keywords:Fertilizer Methane Methanogen Paddy mcrA pmoA


text link:https://www.sciencedirect.com/science/article/pii/S0048969718302754


主站蜘蛛池模板: 兴安盟| 资源县| 桦南县| 淮安市| 阿克| 房产| 富民县| 西平县| 于都县| 都安| 曲麻莱县| 河北省| 德惠市| 安仁县| 侯马市| 富蕴县| 五寨县| 施秉县| 安图县| 喀什市| 枣强县| 东宁县| 历史| 九江县| 衡阳县| 黄梅县| 绩溪县| 鹿泉市| 同仁县| 河津市| 甘洛县| 乡城县| 凤山县| 霍山县| 丰宁| 怀集县| 中宁县| 五常市| 织金县| 镇平县| 宁远县|