国产青榴社区91精品,久久成人精品免费播放,久久精品人人做人人试看

Home> Market activity > Achievements > article shows > article details

Comparative transcriptomic analysis provides insights into the molecularbasis of the metamorphosis and nutrition metabolism change from zoeaeto megalopae inEriocheir sinensis

2016-02-15



Abstract:


Within the larval period of Eriocheir sinensis, there is pronounced morphological changes upon the molt from the

fifth zoeae (Z5) to megalopae (M), and low survival rate exists during this transition, which is typical in crab species. RNA sequencing was applied to Z5 and M of E. sinensis, resulting in the discovery of 19,186 unigenes and 652 differentially expressed genes (DEGs, 3.40% of the unigenes). The important metabolic pathways that might play roles in the larval development of E. sinensis from Z5 to M were detected to be 'Xenobiotics Biodegradation and Metabolism (8.16%)', 'Metabolism of Cofactors and Vitamins (6.70%)', 'Lipid Metabolism (6.36%)', and 'Amino Acid Metabolism (6.28%)'. Further, 19 DEGs possibly contributing to the morphological and sensory capability changes of the larvae were identified, like multiple copies of cuticle protein genes, retinaldehyde-binding protein1 (RLBP1), envelope protein (Envelope) and hormone-related gene ecdysteroid-regulated 16 kDa protein(ESR16). Moreover,62DEGs were identified to be related to carbohydrate,lipid and protein digestion and metabolism, such as glucose dehydrogenases (GDHs), lipases (LIPs) and serine proteases (SPs). Among these DEGs, more genes related to the substance metabolism were found up-regulated in Z5 than M, suggesting that more

energy might be essential to be released for Z5 to complete the transition into M. Characterization of the crucial DEGs by real-time quantitative PCR re-conformed their expression pattern. This study provides the first genome-wide transcriptomic analysis of E. sinensis Z5 and M for studying the molecular basis of the larvae metamorphosis and nutrition metabolism.


主站蜘蛛池模板: 仲巴县| 阿克苏市| 赤城县| 班戈县| 桓仁| 安泽县| 东兰县| 昌邑市| 潍坊市| 宁化县| 化州市| 垫江县| 涿鹿县| 九龙坡区| 乌海市| 辉南县| 巴马| 延长县| 贵港市| 高碑店市| 广州市| 襄垣县| 洛浦县| 车险| 新乡市| 德令哈市| 江孜县| 方城县| 库尔勒市| 大厂| 陵水| 乐昌市| 凉城县| 长治县| 疏附县| 福安市| 丰顺县| 财经| 连州市| 屯留县| 昌邑市|